Table 1. Primers used for reverse transcription-quantitative PCR.
Gene | Primer sequence (5′-3′) |
---|---|
miR-19b-3p | F: ACTGAGTCGTATCCAGTGCAA |
R: GTATCCAGTGCGTGTCGTGG | |
U6 | F: CTCGCTTCGGCAGCACA |
R: AACGCTTCACGAATTTGCGT | |
PTEN | F: TGGATTCGACTTAGACTTGACCT |
R: GGTGGGTTATGGTCTTCAAAAGG | |
MMP-2 | F: TACAGGATCATTGGCTACACACC |
R: GGTCACATCGCTCCAGACT | |
E-cadherin | F: CGAGAGCTACACGTTCACGG |
R: GGGTGTCGAGGGAAAAATAGG | |
Vimentin | F: GACGCCATCAACACCGAGTT |
R: CTTTGTCGTTGGTTAGCTGGT | |
GAPDH | F: GGAGCGAGATCCCTCCAAAAT |
R: GGCTGTTGTCATACTTCTCATGG |