Fig. 1.

SARS-CoV-2 kinetics in bovine, ovine, and swine respiratory EVOCs. Viral titers expressed as Log₁₀ TCID₅₀/mL of SARS-CoV-2 increased significantly in bovine and ovine EVOCs, suggesting viral growth, from 1 to 72 hpi (p < 0.05). In porcine EVOCs, SARS-CoV-2 titers did not change over time. In bovine tissues, D614G replicated significantly at higher magnitude compared to D614 at 48 hpi (p < 0.05) in tracheal EVOCs; 48 hpi (p <0.01) and 72 hpi (p < 0.05) in lung EVOCs. In ovine tissues, both viruses replicated at comparable levels. EVOCs were infected with a dose of 10³ TCID₅₀/mL of each SARS-CoV-2 isolate. Titer values represent the mean of three replicates of three independent experiments. Bars indicate standard deviation (SD). * (p < 0.05), ** (p < 0.01), *** (p < 0.001).