Figure 3.

Effects of target metabolites on the cell growth rates of SW480 colorectal cancer cells and SW480 transfectants. SW480 cells were grown at 5 × 10⁴/well in 96‐well plates for 24 h then exposed to the indicated concentrations of inositol (A), ribitol (B), 2‐hydroxybutyric acid (C), 3‐hydroxypropanoic acid (D), and sarcosine (E). After 24 h, the rate of cell growth was evaluated. Data are shown as the mean ± SD, n = 3–10. Statistical significance was analyzed using Student's t‐test, and a level of probability of 0.05 was used as the criterion of significance. *P < 0.05. L‐proline (0.6–1.8 mM), arabitol (0.1–10 mM), and isoleucine (0.6–1.8 mM) did not significantly affect the rate of cell growth (data not shown).