Table 2.

Metabolites that showed significantly altered tissue levels between APC ^min/+ mice and wild‐type mice

	Chemical class	Fold induction			P‐value
		Polyp/control	Polyp/non‐polyp	Non‐polyp/control	Polyp versus control	Polyp versus non‐polyp	Non‐polyp versus control
1,5‐Anhydro‐d‐glucitol	Carbohydrates	0.41	1.35	0.30	0.00120*	0.20000	0.00011*
1,6‐Anhydroglucose	Carbohydrates	2.18	1.60	1.36	0.02900*	0.17000	0.33000
1‐Methylhistamine	Cyclic amines	2.59	1.91	1.35	0.08500	0.17000	0.04100*
2,3‐Bisphospho‐glycerate	Acyl phosphates	0.28	0.55	0.52	0.27000	0.04700*	0.46000
2‐Aminobutyric acid	Amino acids	9.70	3.60	2.69	<0.00001*	<0.00001*	0.00540*
2‐Hydroxypyridine	Hydroxy acids	1.41	0.74	1.90	0.06200	0.04100*	0.00710*
3‐Hydroxy‐butyrate	Hydroxy acids	2.47	1.43	1.73	0.00015*	0.04700*	0.04100*
4‐Aminobutyric acid	Amino acids	1.22	0.57	2.14	0.13000	0.00250*	0.00077*
4‐Hydroxybenzyl alcohol	Alcohols and polyols	1.26	0.72	1.76	0.12000	0.06000	0.01100*
5‐Hydroxyindoleacetate	Indoles and indole derivatives	2.38	1.42	1.67	0.03200*	0.23000	0.01200*
Adenine	Purines and purine derivatives	1.95	1.65	1.18	0.02800*	0.05300	0.40000
Arabitol	Alcohols and polyols	0.82	0.64	1.29	0.32000	0.03800*	0.09800
Ascorbic acid	Hydroxy acids	3.91	5.80	0.67	0.01400*	0.00290*	0.59000
α‐Sorbopyranose (or fructose)	Carbohydrates	1.77	1.26	1.40	0.00210*	0.14000	0.05400
β‐Alanine	Amino acids	1.30	1.06	1.22	0.02400*	0.56000	0.20000
β‐Glutamic acid	Amino acids	0.23	0.29	0.79	0.00003*	0.00330*	0.20000
Ciliatine	Amino acids	0.12	0.16	0.75	0.00330*	<0.00001*	0.32000
Citric acid + isocitric acid	Tricarboxylic acids	8.18	4.60	1.78	0.16000	0.21000	0.02600*
Cystathionine	Amino acids	14.67	5.34	2.75	<0.00001*	0.00001*	0.01300*
Cysteine + cystine	Amino acids	0.44	0.44	0.99	0.00480*	0.00100*	0.97000
Dimethylbenzimidazole	Heterocyclic aromatic organic compound	3.34	2.13	1.57	0.00250*	0.02100*	0.19000
Fructose	Carbohydrates	1.82	1.27	1.43	0.00160*	0.12000	0.04000*
Fructose‐6‐phosphate	Sugar phosphates	3.67	1.32	2.78	0.01400*	0.42000	0.01200*
Fucose	Carbohydrates	2.34	1.52	1.55	<0.00001*	0.00450*	0.02600*
Fumaric acid	Dicarboxylic acids	0.65	0.62	1.05	0.01000*	0.06100	0.81000
Galactitol	Alcohols and polyols	2.90	1.00	2.90	0.02100*	1.00000	0.08900
Galactosamine	Carbohydrates	0.68	0.59	1.14	0.17000	0.00058*	0.56000
Galactose	Carbohydrates	0.78	0.59	1.32	0.19000	0.01800*	0.17000
Galacturonic acid	Hydroxy acids	0.61	0.71	0.87	0.05000	0.04400*	0.52000
Glucosamine	Aminoglycosides	3.22	1.25	2.59	0.27000	0.79000	0.27000
Glucose	Carbohydrates	0.53	0.61	0.88	0.01600*	0.04900*	0.56000
Glucuronate	Carbohydrates	0.53	0.62	0.86	0.01300*	0.03800*	0.51000
Glutaric acid	Dicarboxylic acids	0.79	0.49	1.61	0.16000	0.00260*	0.00750*
Glyceraldehyde	Alcohols and polyols	0.88	0.68	1.30	0.50000	0.04600*	0.22000
Glyceric acid	Hydroxy acids	1.20	0.84	1.43	0.10000	0.21000	0.02000*
Glycerol	Alcohols and polyols	0.45	0.68	0.66	0.00007*	0.09800	0.03700*
Homoserine	Amino acids	9.43	2.95	3.20	<0.00001*	<0.00001*	0.00007*
Hypotaurine	Amino acids	2.53	2.61	0.97	0.01600*	0.01400*	0.90000
Hypoxanthine	Purines and purine derivatives	3.49	6.80	0.51	0.00007*	0.00001*	0.08900
Inositol	Alcohols and polyols	0.77	0.65	1.18	0.01800*	0.02400*	0.30000
Isoleucine	Amino acids	0.88	0.66	1.33	0.52000	0.02700*	0.17000
Kojic acid	Hydroxy acids	2.90	1.74	1.67	0.00260*	0.02200*	0.13000
Malic acid	Dicarboxylic acids	0.69	0.62	1.10	0.00210*	0.04700*	0.63000
N‐α‐acetyl‐l‐lysine	Amino acids	0.59	0.55	1.07	0.07000	0.03500*	0.79000
N‐acetyl‐d‐glucosamine	Carbohydrates	1.16	0.79	1.47	0.43000	0.17000	0.01500*
O‐phosphoethanolamine	Acyl phosphates	2.43	1.05	2.33	0.00250*	0.85000	0.01400*
Ornithine	Amino acids	0.56	0.58	0.98	0.00700*	0.01400*	0.89000
Orotic acid	Pyrimidines and derivatives	3.35	1.51	2.22	0.00008*	0.03400*	0.00260*
Oxalate	Dicarboxylic acids	1.20	0.77	1.55	0.15000	0.09200	0.01300*
Pantothenate	Amino alcohols	0.50	0.47	1.07	0.00470*	0.00087*	0.70000
Putrescine	Polyamines	14.01	13.63	1.03	0.04700*	0.04700*	0.95000
Rhamnose	Carbohydrates	2.80	1.37	2.05	<0.00001*	0.08700	0.01600*
Ribose	Carbohydrates	0.42	0.42	1.00	0.00120*	0.00150*	1.00000
Ribulose	Carbohydrates	0.65	0.57	1.13	0.01600*	0.01000*	0.43000
Sarcosine	Amino acids	10.44	2.08	5.01	<0.00001*	0.00075*	<0.00001*
Sorbitol	Alcohols and polyols	0.62	0.71	0.86	0.04100*	0.08100	0.49000
Succinic acid (or aldehyde)	Dicarboxylic acids	0.53	0.63	0.85	0.03600*	0.09800	0.55000
Tagatose	Carbohydrates	2.73	1.95	1.40	0.00030*	0.00630*	0.18000
Trans‐4‐hydroxy‐L‐proline	Amino acids	4.30	2.00	2.15	<0.00001*	0.00011*	0.00100*
Uric acid	Purines and purine derivatives	0.73	0.37	1.95	0.03300*	0.00039*	0.00200*
Xylitol	Alcohols and polyols	0.45	0.52	0.86	0.00160*	0.00620*	0.42000

Values are represented as the fold‐induction of the peak intensity among polyps (n = 7) and non‐polyp tissues (n = 7) of the APC^min/+ mice, and the corresponding control intestinal tissues (n = 6) of the wild mice. All data are represented in Table S2, and the metabolites that showed significantly altered levels are shown in Table 2. APC^min, APC multiple intestinal neoplasia. P‐values were calculated using Student's t‐test; *P < 0.05.