Table 6.
Identified major metabolites in sera of APC min/+ mice and SW480 cell‐implanted nude mice
| Pathway and chemical class | Metabolite | Fold induction (APCmin/+ mice) | Fold induction (SW480 cell‐implanted nude mice) | ||
|---|---|---|---|---|---|
| APCmin/+ mice/wild mice | Truncated APC/full‐length APC | Full‐length APC/control | Truncated APC/control | ||
| Glycolysis | Glucose | 1.16 | 1.23* | 0.87 | 1.08 |
| Fructose‐6‐phosphate | 0.63 | n.d. | n.d. | n.d. | |
| Pyruvate + oxalacetic acid | 1.05 | 0.89 | 0.76 | 0.68 | |
| Lactic acid | 1.18 | 1.01 | 0.70** | 0.70** | |
| Tricarboxylic acid cycle | Citric acid + isocitric acid | 1.12 | 0.97 | 0.96 | 0.93 |
| Succinic acid | 1.08 | 0.87 | 0.83 | 0.72* | |
| Fumaric acid | 0.76 | 0.83 | 0.92 | 0.76 | |
| Malic acid | 0.84 | 0.75 | 0.89 | 0.66 | |
| Amino acids (essential) | Histidine | 2.04* | 0.90 | 0.77** | 0.69** |
| Isoleucine | 1.39 | 1.31 | 0.79 | 1.04 | |
| Lysine | 3.08*** | 0.89 | 0.82* | 0.73** | |
| Methionine | 1.26 | 1.11 | 0.87 | 0.96 | |
| Phenylalanine | 1.34* | 1.12 | 0.87 | 0.97 | |
| Threonine | 1.31 | 1.16 | 0.82 | 0.95 | |
| Tryptophan | 0.37* | 0.93 | 1.26 | 1.17 | |
| Valine | 1.34* | 1.17 | 0.85 | 1.00 | |
| Amino acids (non‐essential) | Alanine | 2.01*** | 1.02 | 0.72** | 0.74* |
| Asparagine | 1.98** | 0.81 | 0.82 | 0.67* | |
| Aspartic acid | 1.08 | 0.78 | 1.25 | 0.97 | |
| Cysteine + cystine | 1.79*** | 1.08 | 0.90 | 0.98 | |
| Glutamic acid | 0.72 | 0.70 | 1.31 | 0.92 | |
| Glutamine | 1.35* | 1.00 | 0.82* | 0.81 | |
| Glycine | 1.18 | 1.06 | 0.86 | 0.92 | |
| Proline | 2.68*** | 0.98 | 0.82 | 0.81 | |
| Serine | 1.25 | 0.99 | 0.88 | 0.88 | |
| Tyrosine | 1.46** | 0.94 | 0.87 | 0.82 | |
| Monosaccharides | Allose | 0.50 | n.d. | n.d. | n.d. |
| Arabinose | 1.21 | 0.75 | 1.14 | 0.86 | |
| Fructose | 0.60*** | 1.03 | 1.18 | 1.21 | |
| Fucose | 0.85 | n.d. | n.d. | n.d. | |
| Galactose | 0.78** | n.d. | n.d. | n.d. | |
| Glucosamine | 1.78** | n.d. | n.d. | n.d. | |
| Glucose | 1.16 | 1.23* | 0.87 | 1.08 | |
| Glucuronate | 0.65*** | 1.04 | 0.92 | 0.95 | |
| Gulcono‐1,4‐lactone | 1.25 | n.d. | n.d. | n.d. | |
| Mannose | 1.21* | 0.97 | 0.90 | 0.87 | |
| Rhamnose | n.d. | 2.07* | 0.57 | 1.18 | |
| Ribose | 0.28*** | 0.66** | 1.34* | 0.88 | |
| Ribulose | n.d. | 0.52 | 1.11 | 0.57 | |
| Tagatose | n.d. | 1.15* | 0.75 | 0.86 | |
| Xylulose | 0.58** | n.d. | n.d. | n.d. | |
| Xylose | 1.87 | 0.99 | 0.43* | 0.43* | |
| Alcohols | 2‐Aminoethanol | 0.95 | 0.95 | 0.93 | 0.88 |
| Arabitol | 0.27*** | 0.55* | 3.67*** | 2.01* | |
| Inositol | 1.13 | 0.90 | 1.03 | 0.93 | |
| Lyxose(or xlylose) | 1.52 | n.d. | n.d. | n.d. | |
| Ribitol | 0.45*** | 0.27* | 1.69 | 0.46 | |
| Xylitol | 0.42*** | 0.88 | 1.10 | 0.96 | |
| Nucleobases | Adenine | 2.01*** | n.d. | n.d. | n.d. |
| Thymine | 3.20** | n.d. | n.d. | n.d. | |
| Uracil | 1.74*** | 1.11 | 0.69** | 0.76* | |
Alterations in serum levels of metabolites were shown according to major metabolomic pathways and chemical classes. Values are represented as the fold‐induction between two groups shown in Table 6. APCmin, APC multiple intestinal neoplasia. P‐values were calculated using Student's t‐test; *P < 0.05; **P < 0.01; ***P < 0.001. n.d., not determined.