Figure 4.
Effect of radiation therapy/resveratrol (XRT/RSV) on apoptosis of PC‐3 cells evaluated 72 h after XRT. (a) Representative TUNEL staining of PC‐3 cells. Original magnification, ×400. (b) TUNEL+ cells (brown) in five to six randomly selected high power fields of three slides were counted. (c) Cellular caspase‐3 activity was measured using a caspase‐3/CPP32 colorimetric assay kit. Results are expressed as mean caspase‐3 activity relative to controls + SEM. Assays were done in triplicate. *P < 0.05, significant difference in the percentage of TUNEL+ cells in the groups treated with XRT or XRT/RSV versus controls. #P < 0.05, significant difference in the percentage of TUNEL+ cells between the group treated with XRT/RSV versus the group treated with XRT alone. Figures are representative of two or three independent experiments.