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. Author manuscript; available in PMC: 2020 Nov 24.
Published in final edited form as: Cell Rep. 2020 Nov 10;33(6):108361. doi: 10.1016/j.celrep.2020.108361

Figure 3. IsoTarget Uncovers Redundant Functions for Dscam[TM1] and [TM2] in Dendrite Self-Avoidance.

Figure 3.

(A–D) Dscam[TM1] and [TM2] function redundantly in mediating dendritic self-avoidance in C3 da neuron. In third-instar larvae, C3 da dendrites rarely fasciculate or intersect with each other in WT (A), whereas the loss of both Dscam[TM1] and [TM2] isoforms (Dscam[TM1/2]iso-KO) significantly impairs self-avoidance (B). Loss of either Dscam [TM1] (C) or [TM2] (D) does not affect dendritic self-avoidance. The red arrows point to dendritic crossing sites.

(E) Quantification of dendritic branch crossings in the C3 da neuron ddaF.

(F–I) Dscam[TM1] and [TM2] function redundantly in mediating dendritic self-avoidance in C4 da neurons. The MARCM technique was used. Small red arrows point to crossings of fine dendritic branches, and large red arrows point to crossings of major dendritic branches, which are only observed when both isoforms are lost.

(J) Quantification of dendritic branch crosses in the C4 da neuron ddaC.

See also Figure S4.