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. Author manuscript; available in PMC: 2020 Nov 24.
Published in final edited form as: Cell Rep. 2020 Nov 10;33(6):108361. doi: 10.1016/j.celrep.2020.108361

KEY RESOURCES TABLE

REAGENT or RESOURCE SOURCE IDENTIFIER
Antibodies
rabbit anti-RFP Rockland RRID: AB_2209751
mouse anti-V5 Invitrogen RRID: AB_2556564
chicken anti-GFP Aves Laboratories RRID: AB_2307313
mouse anti-GFP Invitrogen RRID: AB_221568
rat anti-HA Roche RRID: AB_390918
rat anti-Elav DSHB RRID: AB_528218
mouse anti-Dscam (cytoplasmic domain) Shi et al., 2007 N/A
mouse anti-Dscam (ectodomain) Hattori et al., 2009 N/A
rabbit anti-Dock Gift from Jack Dixon; Schmucker et al., 2000 N/A
rabbit anti-Wnd Collins et al., 2006 N/A
anti-rabbit Rhodamine RX Jackson ImmunoResearch RRID: AB_2340613
anti-mouse Alexa Fluor 488 Jackson ImmunoResearch RRID: AB_2340846
anti-chicken Alexa Fluor 488 Jackson ImmunoResearch RRID: AB_2340375
anti-rat Alexa Fluor 488 Jackson ImmunoResearch RRID: AB_2749829
anti-mouse HRP Jackson ImmunoResearch RRID: AB_10015289
anti-rat HRP Jackson ImmunoResearch RRID: AB_2338128
anti-rabbit HRP Jackson ImmunoResearch RRID: AB_10015282
Experimental Models: Drosophila Strains
Dscam[TM1]-RSRT-tlstop-RSRT-HA This study N/A
Dscam[TM1]-RSRT-short tlstop-RSRT-HA This study N/A
Dscam[TM2]-RSRT-tlstop-RSRT-V5 This study N/A
RDL[RG]-RSRT-tlstop-RSRT-V5 This study N/A
RDL[RF]-RSRT-tlstop-RSRT-V5 This study N/A
RDL-RSRT-tlstop-RSRT-V5 Hu et al., 2020 N/A
UAS-Dock-OLLAS This study N/A
ppk-GAL4 Grueber et al., 2007 N/A
GMR83B04-GAL4 Pfeiffer et al., 2008 N/A
UAS-Dscam[TM2]::GFP (#1, 3.36.25, chromosome X) Wang et al., 2004 N/A
UAS-Dscam[TM2]::GFP (#2, 3.36.25, chromosome III) Wang et al., 2004 N/A
UAS-Dscam[TM1]::GFP (3.36.25, chromosome X) Wang et al., 2004 N/A
DscamP-Dscam[TM1]::GFP (3.36.25) Wang et al., 2004 N/A
DscamP-Dscam[TM2]::GFP (3.36.25) Wang et al., 2004 N/A
Dscam18 Wang et al., 2002 N/A
HiwΔN Wu et al., 2005 N/A
UAS-Wnd Collins et al., 2006 N/A
UAS- GFP-WndKD Xiong et al., 2010 N/A
GAL4-OK107 Bloomington BL854
dockC506 Bloomington BL43376
dock4732 Bloomington BL10444
Oligonucleotides
Dscam[TM1] forward primer: 5’-CGTTACCGGAGGCACTATCG-3’ This study N/A
Dscam[TM1] reverse primer: 5’- ATCGTCTTTGTGGTGATTGCC-3’ This study N/A
Dscam[TM2] forward primer: 5’- CGTTACCGGAGGCACCATT-3’ This study N/A
Dscam[TM2] reverse primer: 5’- ACTACATCGTAGTACACATCCTTT-3’. This study N/A
Chmp1 forward primer: 5’-AAAGGCCAAGAAGGCGATTC-3’ Kim et al., 2013 N/A
Chmp1 reverse primer: 5’-GGGCACTCATCCTGAGGTAGTT-3’ Kim et al., 2013 N/A
Elav forward primer: 5’-CTGCCAAAGACGATGACC-3’ Kim et al., 2013 N/A
Elav reverse primer: 5’-TAAAGCCTACTCCTTTCGTC-3’ Kim et al., 2013 N/A
Dscam exon 16–18 forward primer 1: 5’-TCGAACAACG TGAAGCCCGATAACA-3’ This study N/A
Dscam exon 16–18 Reverse primer 1: 5’-CCTCGCTTAATCCGGTCACAGGTA −3’ This study N/A
Dscam exon 16–18 forward primer 2: 5’-TGGTACAACCTCCGCATCAC-3’ This study N/A
Dscam exon 16–18 Reverse primer 2: 5’-TATAGTTGGATCCGGGAACGG-3’ This study N/A
Software and Algorithms
Prism 8 GraphPad RRID:SCR_002798
Fiji NIH RRID:SCR_002285
Leica Microsystems LAS AF (version 2.6.3) Leica Microscopy RRID:SCR_008960
Neurolucida MBF Bioscience RRID:SCR_001775
Neurolucida Explorer MBF Bioscience RRID:SCR_017348