FIGURE 5.

HMGB1 is a direct target of miR‐141–3p. On‐line bioinformatics algorithms (Targetscan; www.targetscan.org) revealed that a conserved sequence in the 3'‐UTR of HMGB1 could be bonded with miR‐141–3p (A). A direct interaction between miR‐141–3p and the 3'‐UTR of HMGB1 was analyzed using a luciferase reporter assay (B) (n = 3 in each group.). After transfection with miR‐con or miR‐141–3p mimics into MDA‐MB‐231 and MCF‐7 for 48 hours, protein expression of HMGB1 (C) and HIF‐1α (D) was measured using western blotting (n = 3 in each group.). Protein expression of HMGB1 was detected using IHC staining in 74 pairs of adjacent non‐tumor tissues and BC tissues (E) (n = 74 in each group.). Pearson's correlation analysis showed that the expression of HMGB1 was significantly and inversely correlated with miR‐141–3p in BC tissues (F) (n = 74 in each group.). ^* p < 0.05 compared to the corresponding control group