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. 2020 Sep 10;64(20):2000108. doi: 10.1002/mnfr.202000108

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© 2020 The Authors. Published by Wiley‐VCH GmbH

This is an open access article under the terms of the http://creativecommons.org/licenses/by-nc/4.0/ License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited and is not used for commercial purposes.

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BE influence on vesiculation‐related gene expression in the presence of bzATP and P2X₇ antagonist. BzATP increased A) Rab27b, B) Rab27a, and C) SMPD1 expression in HUVECs via P2X₇‐dependent mechanism. BE decreased basic expression of Rab27b (A) and Rab27a (B). Pre‐incubation with BE was able to inhibit the effect of bzATP on Rab27b, Rab27a, and SMPD1 genes. D) ARF6 expression following the treatments did not present any pattern. Error bars represent SD. One‐way ANOVA with FDR correction, n = 4. *Statistically significant, p < 0.05.