Figure 7.

Proposed molecular mechanisms of BE‐induced reduction of vesicle release. Previous results demonstrated that anthocyanins (AC) enter ECs via bilitranslocases (1) and inhibit PI3K activation (2). This prevents Akt phosphorylation and reduces P2X₇ transcription (3) that stands in line with our results. According to the literature, P2X₇ signaling pathway leads to Akt phosphorylation and further EV release (4). Moreover, our data shows that P2X₇ cascade also augments transcription of vesiculation‐related genes Rab27b and Rab27a that are inhibited by BE treatment (5), possibly due to a decrease in P2X₇ expression. We propose that P2X₇‐evoked increase in Rab27b and Rab27a transcription reflects increased cellular demands after P2X₇‐stimulated vesiculation.