FIGURE 3.

Colony formation and invasiveness of Ode macrophages after RASGRP1 and GZMA knockdown. (a,b) qRT‐PCR confirmation of GZMA and RASGRP1 knockdown, respectively. (c) Matrigel chamber assay showing a regain in matrigel traversal after RASGRP1 and GZMA knockdown. (d) Increased colony formation in soft agar following RASGRP1 knockdown. Non‐transfected virulent disseminating Ode macrophages are indicated by V, and non‐transfected poorly disseminating attenuated Ode macrophages by A. Error bars represent SEM of three biological replicates. ****p < .0005, ***p < .001, **p < .005 and *p < .05