Figure 4.
Runx1 is required for the regulation of critical genes to maintain functional AMPs for leukemogenesis by Cbfb-MYH11. (A-G) RNA-seq was performed on AMP cells isolated from Mx1-CreCbfb+/56M and Runx1f/fMx1-CreCbfb+/56M mice 2 to 3 weeks after pIpC treatment. N = 3 for each genotype. (A) Two-dimensional principal component analysis plots showing clear separation between these 2 genotype groups. (B) Heatmap representation of identified DEGs between these 2 groups. Numbers and percentages of DEGs in each of the 2 expression clusters (down- and upregulated) are listed on the left. (C-E) GSEA (Preranked) identified curated genes sets significantly enriched in these DEGs, including (C) the FISCHER_G2_M_CELL_CYCLE gene set, which is negatively correlated with DEGs upregulated in Runx1f/fMx1-CreCbfb+/56M cells; (D) the BROWN_MYELOID_CELL_DEVELOPMENT_UP gene set, which is positively correlated with DEGs upregulated in Runx1f/fMx1-CreCbfb+/56M cells; and (E) the GAL_LEUKEMIC_STEM_CELL_DN gene set, which is positively correlated with DEGs upregulated in Runx1f/fMx1-CreCbfb+/56M cells.