Table 2.

IHC protocols.

Antibody	Host	Clone	Dilution	Antigen retrieval	Source
CD4	Rabbit	EPR6855	1:400	AC	Abcam
CD8	Mouse	M7103	1:50	AC	Dako
CD68	Mouse	M0876	1:100	Trypsin	Dako
CD163	Mouse	M3527	1:600	AC	Dako
S100p	Rabbit	–	1:9,000	–	Dako
CD31	Mouse	M0823	1:100	AC	Dako
Ki67	Mouse	M7240	1:100	AC	Dako
TH	Mouse	T1299	1:2,000	MW	Sigma
DDC	Rabbit	AB136	1:500	–	Millipore
PNMT	Mouse	Ab119784	1:2,000	–	Abcam

AC, autoclave 121°C 5 min, pH = 6 buffer; MW, microwave 500 W 20 min, pH = 6 buffer.