a, Diagram of the experimental design. Objects are not drawn to scale. Representative infrared fundus image (b, d, f, h) and B-scan OCT images (c, e, g, i) of mouse eyes intravitreally (b, c, d, e) or subretinally (f, g, h, i) injected with 2 μl of 0.97 nM Ana GVs. Images of the same location of the eyes collected before (b, c, f, g) or after (d, e, h, i) ultrasound treatment. The arrows indicate the vitreous or subretinal space, where contrast from gas vesicles was expected. Scale bars represent 0.5 mm.