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. Author manuscript; available in PMC: 2021 Nov 3.
Published in final edited form as: Cell Metab. 2020 Nov 3;32(5):736–750.e5. doi: 10.1016/j.cmet.2020.10.007

Figure 1. Membrane-associated PK is sufficient to close KATP channels.

Figure 1.

(A) Experimental setup for excised patch of KATP channels.

(B) Applying the substrates for PK closes KATP channels in mouse islets. ATP, 1 mM ATP; ADP, 0.5 mM ADP + 0.1 mM ATP; PEP, 5 mM phosphoenolpyruvate. Holding potential, −50 mV.

(C-F) Analysis of KATP channel closure in terms of power (C), frequency (D), open time (E), and amplitude (F) in mouse islets.

(G) Applying the substrates for PK closes KATP channels in human islets.

(H-K) Analysis of KATP channel closure in terms of power (H), frequency (I), open time (J), and amplitude (K) from 3 human islet donors.

Data are shown as mean ± SEM. *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001 by 1-way ANOVA. See also Figure S1 and Table S1.