Skip to main content
. Author manuscript; available in PMC: 2021 Nov 3.
Published in final edited form as: Cell Metab. 2020 Nov 3;32(5):736–750.e5. doi: 10.1016/j.cmet.2020.10.007

Figure 3. PK amplifies insulin secretion by a distinct mechanism from glucokinase.

Figure 3.

(A) Glucose-dose response of insulin secretion in human islets (n = 8) in the absence or presence of GKa and PKa.

(B-E) Representative recordings and quantification of cytosolic calcium and ATP/ADP oscillations (duty cycle and period) in mouse islets. (B) Glucose elevation from 10 mM glucose (10G) to 13 mM glucose (13G) (n = 51). (C) Glucose reduction from 10G to 8 mM glucose (8G) (n = 53). (D) Addition of 500 nM GK activator (GKa) RO-0281675 in 10 mM glucose (n = 37).

(E) Addition of 10 μM PK activator TEPP-46 in 10 mM glucose (n = 62 islets from 4 mice). FuraRed (Ca2+), black scale bar = 0.01 (B, D) or 0.1 (C, E) R430/500; Perceval-HR (ATP/ADP), red scale bar = 0.01 R500/430.

Data are shown as mean ± SEM. *P < 0.05, ***P < 0.001, ****P < 0.0001 by 1-way ANOVA (A) or paired Student’s t-test (B-E). See also Table S1.