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. Author manuscript; available in PMC: 2020 Nov 24.
Published in final edited form as: Nat Microbiol. 2018 Dec 10;4(2):362–374. doi: 10.1038/s41564-018-0318-0

Fig. 6 |. The NLRP3 inflammasome mediates lethality induced by B. cereus infection in vivo.

Fig. 6 |

(a) Survival of WT, Nlrp3–/– and Casp1/11–/– mice, after intraperitoneal infection with 5 × 106 colony-forming units (CFUs) of B. cereus. (b) Concentration of IL-18 in the serum of WT, Nlrp3–/– and Casp1/11–/– mice, 16 hr after intraperitoneal infection with 7.5 × 106 CFUs of B. cereus. (c) Concentration of IL-18 in the serum of WT, Nlrp3–/– and Casp1/11–/– mice, 16 hr after intraperitoneal injection with 200 μl of the supernatant of B. cereus (Sup). (d) Survival of WT mice after intraperitoneal injection with 5 × 106 CFUs either of B. cereus ATCC 10876 or of ΔHbl B. cereus (ΔHbl). (e) Concentration of IL-18 in the peritoneal fluid of WT mice, 3 hr after intraperitoneal infection with 7.5 × 106 CFUs either of B. cereus or of ΔHbl B. cereus. (f) Concentration of IL-18 in the serum of WT mice, 4 hr after intraperitoneal infection with 7.5 × 106 CFUs either of B. cereus or of ΔHbl B. cereus. (g) Survival of WT mice administered either with PBS or with MCC950, both via an intraperitoneal route, followed by intraperitoneal infection with 5 × 106 CFUs of B. cereus with a corresponding second dose of PBS or MCC950. (h) Concentration of IL-18 in the peritoneal fluid of WT mice administered with PBS or WT mice administered with MCC950 as in (g), 3 hr after infection with 7.5 × 106 CFUs of B. cereus. (i) Concentration of IL-18 in the serum of WT mice administered either with PBS or with MCC950 as in (g), 6 hr after infection with 7.5 × 106 CFUs of B. cereus. Each symbol represents an individual mouse (b, c, e, f, h, i). **P < 0.01, ***P < 0.001 and ****P < 0.0001 (two-sided log-rank test [a, d and g] or one-way ANOVA with Dunnett’s multiple-comparisons test [b and c] or two-tailed t-test [e, f, h and i]). Data are pooled from two independent experiments (a, b, and d-i) or from three independent experiments (c, mean and s.e.m. in b, c, e, f, h and i).