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[Preprint]. 2020 Nov 18:2020.11.18.388868. [Version 1] doi: 10.1101/2020.11.18.388868

Table 2.

Protein yields of various RBD constructs using the standard TFF-IMAC-SEC process. Numbers represent yields from independent experiments. RBDs represents the same RBD amino acid sequence as the Sinai construct, while RBDr represents the same RBD amino acid sequence as the Ragon construct.

23BCondition 24BProtein Yield (mg/l) 25BMean (mg/l)
26BX22 (Sinai) 29.6, 30.6, 40.9 33.7 ± 6.2
27BM67 (RBDs-His6) 45.1, 50.1 47.6 ± 3.5
28BX24 (Ragon) 8.1, 8.3, 8.6, 11.9, 12.2, 12.6, 18.9 29B11.5 ± 3.8
30B
30BM68 (RBDr-3C-His8-SBP)
58.5, 58.7, 73.6 31B63.6 ± 8.7
32BM69 (RBDr-3C-His8) 45.0, 47.7, 55.7 33B49.5 ± 5.6