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. 2020 Nov 20;13:11975–11990. doi: 10.2147/OTT.S279798

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© 2020 Liu et al.

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Long intergenic non-coding RNA 519 (LINC00519) increases metastasis-associated in colon cancer-1 (MACC1) expression by sponging microRNA (miR)-876-3p in tongue squamous cell carcinoma (TSCC) cells. (A and B) MACC1 mRNA and protein expression in SCC-15 and CAL-27 cells transfected with small interfering RNA specific for LINC00519 (si-LINC00519) or a negative control control (si-NC) were measured by quantitative real-time polymerase chain reaction (qRT-PCR) and Western blotting, respectively. (C and D) LINC00519-depleted SCC-15 and CAL-27 cells were further transfected with anti-miR-876-3p or anti-miR-NC, after which MACC1 mRNA and protein expression were measured using qRT-PCR and Western blotting, respectively. (E) The correlation between LINC00519 and MACC1 mRNA expression in the 52 TSCC tissues was analyzed using a Pearson’s correlation analysis. (F) Enrichment of LINC00519, miR-876-3p and MACC1 via bead precipitation with Ago2 antibody was detected using an RNA immunoprecipitation assay.

Note: **P < 0.01.