Figure 8.

Loss of long intergenic non-coding RNA 519 (LINC00519) hinders the growth of tongue squamous cell carcinoma (TSCC) xenograft tumors in vivo. (A) The silencing efficiency of short hairpin RNA specific for LINC00519 (sh-LINC00519) against LINC00519 was analyzed by quantitative real-time polymerase chain reaction (qRT-PCR). (B) One week after cell injection, the tumor volumes was monitored every 4 days and tumor growth curves were plotted. (C) Photographs of tumor xenografts resected from nude mice. (D) Tumor xenografts were resected and weighted. (E and F) The expression of LINC00519 and miR-876-3p in the tumor xenografts was detected by qRT-PCR. (G and H) qRT-PCR and Western blotting were performed to measure metastasis-associated in colon cancer-1 (MACC1) mRNA and protein expression in tumor xenografts, respectively.

Note: **P < 0.01.