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. 2020 Nov 24;15(11):e0234101. doi: 10.1371/journal.pone.0234101

Fig 4. An optimized Notch responsive SPS element.

Fig 4

(A and B) A synthetic SPS-driven promoter, using the (A) complete TP1 binding element or (B) core TP1 binding element, was transfected into HEK293T cells. These two constructs were activated with either wild-type (WT) or ankyrin mutated (R→A) NICDs to observe their dimer responsiveness. Shown are the average +/- SE of n = 4 independent experiments. Student’s t-test was performed to compare the activation between WT and mutant NICDs where * <0.05, ** <0.01, *** <0.001.