Fig 1. PABA and PHB are metabolized to supply quinone for CoQ₁₀ synthesis in S. pombe.

S. pombe wild-type (WT) PR110 cells were pre-cultivated in 10 mL YES medium for 1 day, 1 μg/mL of ¹³C₆-PABA or ¹³C₆-PHB was added to 55 mL of YES media containing 1×10⁵ cells/mL, and the cells were cultivated for 2 days with rotation at 30°C. CoQ₁₀-enriched samples were obtained after separation of lipids by TLC, and samples were subjected to LC-MS and LC-MS/MS (daughter scan) analyses to detect stable isotope-labeled CoQ₁₀.