Fig 5. CoQ₁₀ production by the ppt1-overexpressing strain treated with 10 μg/mL Bz.

WT PR110 cells harboring pREP41 or pREP41-PPT1OR were cultivated in 10 mL PMU containing 0.15 μg/mL thiamine (Thi) for 1 day. Cells were washed three times with distilled water. Thiamine was added to repress the expression of the nmt1 promoter, and 10 μg/mL of Bz and 0.1 μg/mL PABA/PHB were also added to the media containing ~1×10⁶ cells/mL and the cells were cultivated for the indicated time with rotation at 30°C. Gray bars show the CoQ₁₀ content per 50 mL of medium, and white bars show CoQ₁₀ normalized against cell number. Diamonds show cell number. Five micrograms of CoQ₆ was used as an internal standard. Data are represented as the mean ± SD of three measurements. Asterisks on bars denote statistically significant differences (**p<0.01, *p<0.05) relative to the vector control sample without thiamine (Student’s t-test).