Fig 7. Growth and CoQ₁₀ production of yeast growing on the non-fermentable carbon source YEGES following Bz treatment.

(A) S. pombe strains were spotted onto YES or YEGES with or without 100 μg/mL Bz. Cells grown on YES for 1 day were washed three times. A culture with an OD₆₀₀ of 2 was serially diluted from 10⁻¹ to 10⁻⁵ (from left to right), spotted onto agar media, and cultured for 7 days. (B) For the pre-culture, PR110 yeast cells were cultivated in 55 mL of normal YES medium for 1 day, washed three times with pure water, and 100 μg/mL of benzoic acid (Bz) was added to YEGES media containing 2% (w/v) glycerol and 1% ethanol (w/v) instead of 3% glucose (w/v) with an initial cell density of ~1×10⁷ cells/mL, and cells were cultivated for 3 days at 30°C. Gray bars show the CoQ₁₀ content per 50 mL medium, and white bars show CoQ₁₀ normalized against cell number. Five micrograms of CoQ₆ was used as an internal standard for CoQ extraction. Data are represented as the mean ± SD of three measurements. Asterisks on bars denote statistically significant differences (**p<0.01, *p<0.05) relative to YEGES without Bz (Student’s t-test).