| Component | Amount (per reaction, μL) | Final |
|---|---|---|
| pX330 (1 μg/μL) | 1 | 1 μg |
| 10× FastDigest buffer | 2 | 1 × |
| FastDigest BbsI | 1 | 10 U |
| Fast Alkaline Phosphatase | 1 | 1 U |
| ddH2O | 15 | - |
| Total volume | 20 | |
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