| Component | Amount (per reaction, μL) | Final |
|---|---|---|
| Ligation reaction (from Step v above) | 11 | - |
| 10 mM ATP | 1.5 | 1 mM |
| 10× Plasmid-Safe Buffer | 1.5 | 1× |
| Plasmid-Safe DNase | 1 | 10 U |
| Total volume | 15 | |
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