Depiction of the A. vinelandii proteins containing GGDEF, EAL, or HD-GYP domains in the genome of strain DJ. Prediction of the domains was according to Pfam and SMART. Models are not drawn to scale. Large amino acid regions without a predicted domain are also depicted. (A) EAL and HD-GYP domain-containing proteins. (B) Dual GGDEF-EAL domain-containing proteins. (C) GGDEF domain-containing proteins. The star denotes a protein encoded by the A. vinelandii DJ genome but not the AEIV genome. The GGDEF-EAL protein Avin_25190 was originally annotated as having an additional GGDEF domain at the C terminus. However, analysis of the DNA sequence of this open reading frame in both strain DJ and strain AEIV indicated to us that this GGDEF domain constitutes a different open reading frame, which we have named Avin_25191 (for details, see Fig. S1 in the supplemental material).