FIG 3.

Effects on alginate production of mutations in genes involved in synthesizing or degrading c-di-GMP. Alginate quantification in the parental AEIV strain (WT) and in strains with deletion-insertion mutations in 3 putative PDEs (checkered), 9 hybrid DGC-PDEs (striped), and 14 DGCs (dotted). The stars denote proteins with degenerate GGDEF domains. A mutant unable to produce alginate was included as a negative control (algD); means and standard deviations from three independent experiments are shown. Significant differences were analyzed by one-way analysis of variance with Dunnett's post hoc test. Statistical significance is indicated. ****, P < 0.0001. Cells were grown for 48 h in liquid Burk’s-sucrose medium.