FIG 4.

Effect of AvGReg (Avin_00420) overproduction on the alginate produced by A. vinelandii. (A) Quantification of the amount of c-di-GMP (left) and alginate (right) production in strain AEIV and in its derivative overexpressing the DGC AvGReg (DGC⁺). Production of c-di-GMP in the absence of AvGReg (ΔAvin_00420) is also shown. The bars for standard deviations from three independent experiments are shown. Significant differences were analyzed by t test. Statistical significance is indicated. **, P < 0.01; ****, P < 0.0001. (B) Graphic showing the molecular mass distribution of the alginates produced by strains AEIV and DGC⁺. A representative curve for each strain is shown. A. vinelandii strains were cultivated in Burk’s-sucrose medium for 48 h.