Organization of the T6SS loci. The main cluster (MC) and auxiliary clusters (AC-1, AC-2, and AC-3) are shown. Each gene is color-coded according to its predicted role: light blue, outer sheath; dark blue, inner tube; beige, baseplate; light green, inner membrane complex; dark green, adaptor proteins; light pink, spike tip proteins; orange, effector proteins; hot pink, protease; red, immunity proteins; dark gray, cap (PAAR) proteins; light gray, regulator; white, unknown function. Bent black arrows mark promoters, and yellow triangles indicate transposon insertion sites.