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Functional complementation of E. coli MKH13 with BccT1 (WT) and five BccT1 mutants grown in GB (A), DMG (B), or ectoine (C). Strains were grown for 24 h, and the final optical density (OD595) was compared to that of the strain expressing wild-type BccT1 (pBAVP1456). Means and standard errors from at least two biological replicates are shown. Statistics were calculated using Student’s t test (*, P < 0.05; **, P < 0.01; ***, P < 0.001; ****, P < 0.0001).
