TABLE 3.
Primers used in this study
| Use and primer | Sequence (5′–3′)a | Length (bp) |
|---|---|---|
| Expression | ||
| VP1456 Fwd | tgggctagcgaattcgagctTTTGCTGTAAATATGCAATAAAAGTG | 1,740 |
| VP1456 Rev | ggatccccgggtaccgagctTTAGCGGTAAGCGGAAAG | |
| VPA0356 Fwd | tgggctagcgaattcgagctAGCGGCTTTTTGAACATC | 1,671 |
| VPA0356 Rev | ggatccccgggtaccgagctTTAAACCAAACCTTGATCC | |
| Mutagenesis | ||
| BCCT1 TRP203CYS F | CATGTTTCACtgcGGTGTTCACGGTTGGAG | 7,096 |
| BCCT1 TRP203CYS R | GTCGCGCCCATCGCGAGT | |
| BCCT1 TRP208LEU F | TGTTCACGGTctgAGTATTTACGC | 7,096 |
| BCCT1 TRP208LEU R | CCCCAGTGAAACATGGTC | |
| BCCT1 TYR211LEU F | TTGGAGTATTctgGCCCTTGTTGCGTTG | 7,096 |
| BCCT1 TYR211LEU R | CCGTGAACACCCCAGTGA | |
| BCCT1 TRP384LEU F | GTGGGTATCTctgTCTCCGTTTG | 7,096 |
| BCCT1 TRP384LEU R | CAAGCCCAGTAGAACACTG | |
| BCCT1 203208211ALA F | tgcgagtattgcgGCCCTTGTTGCGTTGGCG | 7,096 |
| BCCT1 203208211ALA R | ccgtgaacacccgcGTGAAACATGGTCGCGCC | |
| BCCT1 TRP384ALA F | GTGGGTATCTgcgTCTCCGTTTG | 7,096 |
| BCCT1 TRP384ALA R | CAAGCCCAGTAGAACACTG | |
a
Regions of complementarity for Gibson assembly are indicated by lowercase type.