FIG 1.
Transient intestinal colonization of HaitiV in adult mice following treatment with streptomycin. (A) Schematic of streptomycin treatment and single oral inoculation with HaitiV. Arrowheads indicate peroral treatment with either streptomycin in sodium bicarbonate or oral gavage with bacteria. Three groups of female C57BL/6 mice (n = 5) were inoculated with either 109 CFU of HaitiV, 109 CFU of formalin-inactivated HaitiV, or a vehicle (sodium bicarbonate) control and kept in conventional housing with streptomycin-supplemented drinking water for the indicated time. Fresh fecal pellets were collected daily from all mice, and blood draws were conducted weekly starting on the day of inoculation. (B) Body weights of mice from cohort 1 over the course of this study. (C) Fecal shedding of HaitiV from mice inoculated with HaitiV. Open symbols depict CFU levels below the limit of detection. No CFU of HaitiV could be recovered from fecal pellets from mice inoculated with formalin-inactivated HaitiV or sodium bicarbonate.