TABLE 1.

Strains and plasmid used in the present study

Strain or plasmid	Genotype and relevant characteristicsa	Source or reference
Bacterial strains
A. vinelandii
AEIV (also named E strain)	WT strain	43
ICM01	AEIV derivative carrying a ΔmucR::Gm mutation	This work
ICM09	ICMO1 derivative complemented with WT copy of mucR integrated into its native locus in the chromosome; Gmr Kmr	This work
CN100	AEIV derivative carrying a ΔalgR::Km mutation	This work
Escherichia coli
DH5α	supE44 ΔlacU169 hsdR17 recA1 endA1 gyrA96 relA	45
BL21(DE3)	F− ompT hsdSB(rB− mB−) gal dcm (DE3)	Invitrogen
Plasmids
pJET1.2/Blunt vector	PCR cloning vector; Apr	Thermo Fisher Scientific
pBSL141	Source of the Gmr cassette	49
pBSL128	Source of the Kmr cassette	49
pET-21a(+)	Expression vector	Novagen
pBBR1MCS-2	Cloning vector; Kmr	50
pJG99	pJET1.2/Blunt vector derivative carrying a 2.6-kb fragment containing the mucR gene	This work
pJG100	pJG99 derivative carrying a ΔmucR::Gm mutation; used to construct the mutant ICM01	This work
pJG99-Km	pJG99 derivative carrying a Kmr cassette as a selection marker at a ScaI site of the multiple cloning site; used to construct the complemented strain ICM09	This work
pJG98	pJET1.2/Blunt vector derivative carrying a ΔalgR::Km construction; linearized with ScaI and used to construct the mutant CN100	This work
pETR-3P	pET-21a(+) vector derivative expressing the AlgR protein carrying a His6 tag at the N terminus	This work

^aGm, gentamicin; Gm^r, gentamicin resistance; Ap^r, ampicillin resistance; Km, kanamycin; Km^r, kanamycin resistance.