Fig. 6.

The Jak2/STAT3 cascade participates in BK-induced PGE₂-dependent MMP-9 expression in RBA cells. a, b Cells were treated with or without AG490 (1 μM) or CBE (0.1 μM) for 1 h before exposure to 10 nM BK (a) or 10 μM PGE₂ (b) for the indicated times. After treatment, the conditioned media were collected and analyzed by gelatin zymorgraphy. c Time dependence of BK-stimulated Jak2 and STAT3 phosphorylation, cells were treated with 10 nM BK for the indicated times. Moreover, cells were pretreated with CLC (30 μM) or AG490 (AG, 1 μM) for 1 h and then exposure to 10 nM BK for 3 min. d Cells were pretreated with U0126 (U0, 1 μM), AG490 (AG, 1 μM), or PP1 (1 μM) for 1 h and then exposure to PGE₂ (10 μM) for 3 min. After treatment, the cell lysates were collected and analyzed by Western blotting with anti-phospho-Jak2, anti-phospho-STAT3 or anti-GAPDH as described under Methods. Data are expressed as mean ± SEM (a, b) or mean (c, d) of three independent experiments (N = 3). *P < 0.05; **P < 0.01, as compared with the respective values of cells stimulated with BK (a) or PGE₂ (b) only at the same time, or basal control (c, d). ^#P < 0.05, as compared with the respective values of cells stimulated with BK or PGE₂ only (c, d). The image represents one of three similar experiments