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. 2020 Nov 23;19:424. doi: 10.1186/s12936-020-03498-w

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© The Author(s) 2020

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 1 — Integration of test promoters into the p230p safe harbor locus of Plasmodium yoelii. Plasmids bearing a single test promoter (see inset) driving GFPmut2 expression were linearized and integrated into the pyp230p locus by double homologous recombination. Transgenic parasites were selected by resistance to pyrimethamine via constitutive expression of human dihydrofolate reductase (HsDHFR). Populations containing transgenic parasites were used for all experiments. Promoter sequences were used from pyclag-a (PY17X_1402200), pydd (PY17X_0418900), pylap4 (PY17X_1323300), pytrap (PY17X_1354800), pyuis4 (PY17X_0502200), pylisp2 (PY17X_1004400), pybip (PY17X_0822200), and pbeef1a (PBANKA_1133300)