Fig. 3.

Down-regulated XIST suppressed the viability of KG-1 cells and decreased their resistance to Adriamycin via MYC down-regulation. a miR-29a expression and mRNA expression of XIST and MYC as well as MYC protein expression in KG-1 cells detected by RT-qPCR and Western blot analysis. b CCK-8 assay was used to detect the viability of KG-1 cells. c CCK-8 assay was used to detect the Adriamycin resistance of KG-1 cells. d RT-qPCR was used to examine the relative expression of MRP1 and P-GP in KG-1 cells. e, f Western blot analysis was performed to test the expression of drug-resistant proteins (MRP1 and P-GP) in KG-1 cells. *p < 0.05 vs. KG-1 cells without any treatment. The data were measurement data, and expressed as mean ± standard deviation. Data comparison among multiple groups was conducted by one-way ANOVA. Cell proliferation ability detected by CCK-8 assay at different time points was measured using two-way ANOVA. The experiment was repeated 3 times independently