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. 2020 Nov 23;18:173. doi: 10.1186/s12951-020-00728-w

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© The Author(s) 2020

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 2 — Nanoparticle complex stability and transfection rates. a For the determination of complexation efficacy by agarose gel electrophoresis, 0.5 µg antimiR were complexed with PEI at the different weight ratios. The complexes were mixed and separated on a 2% agarose gel pre-stained with Sybr™ Gold. b Complex stabilities were measured by heparin displacement assay. c PEI/antimiR complex sizes were determined by Dynamic Light Scattering (DLS). d Transmission electron microscopy (TEM) of PEI/antimiR complexes. e,f Uptake of PEI complexes containing Cy3-labeled antimiR was analyzed by flow cytometry and confocal microscopy