Key resources table.
| Reagent type (species) or resource |
Designation | Source or reference |
Identifiers | Additional information |
|---|---|---|---|---|
| Recombinant DNA reagent (Mus musculus) | GeCKO v2 KO Pooled Library | Sanjana et al., 2014, PMID:25075903; Addgene | CAT: 1000000052 | |
| Recombinant DNA reagent (Mus musculus) | Scrambled control shRNA | Sigma MISSION shRNA library | CAT: SHC016-1EA | |
| Recombinant DNA reagent (Mus musculus) | The p27 shRNA | Sigma MISSION shRNA library | CAT: TRCN0000287390 | |
| Recombinant DNA reagent (Mus musculus) | p27-qPCR-FWD primer | This paper | N/A | TCAAACGTGAGAGTGTCTAACG |
| Recombinant DNA reagent (Mus musculus) | p27-qPCR-REV primer | This paper | N/A | CCGGGCCGAAGAGATTTCTG |
| Recombinant DNA reagent (Mus musculus) | Puromycin-qPCR-FWD primer | This paper | N/A | CTGCAAGAACTCTTCCTCACG |
| Recombinant DNA reagent (Mus musculus) | Puromycin-qPCR-REV primer | This paper | N/A | GGGAACCGCTCAACTCGG |
| Recombinant DNA reagent (Mus musculus) | Control non-targeting (NT) sgRNA | This paper | N/A | GCGAGGTATTCGGCTCCGCG |
| Recombinant DNA reagent (Mus musculus) | Nf2 sgRNA KO1 | This paper | N/A | CGAGATGGAGTTCAACTGCG |
| Recombinant DNA reagent (Mus musculus) | Nf2 sgRNA KO2 | This paper | N/A | ATACTGCAGTCCAAAGAACC |
| Recombinant DNA reagent (Mus musculus) | Traf3 sgRNA KO1 | This paper | N/A | GTGCTCGTGCCGGAGCAAGG |
| Recombinant DNA reagent (Mus musculus) | Traf3 sgRNA KO2 | This paper | N/A | TGGCCCTTCAGGTCTACTGT |
| Recombinant DNA reagent (Mus musculus) | Ube2m sgRNA KO1 | This paper | N/A | GCGCAGCTCCGGATTCAGAA |
| Recombinant DNA reagent (Mus musculus) | Ube2m sgRNA KO2 |
This paper | N/A | GAGTCGGCCGGCGGCACCAA |
| Recombinant DNA reagent (Mus musculus) | Nfkb2/p100 sgRNA KO1 | This paper | N/A | CTGAGCGTGATAAATGACGT |
| Recombinant DNA reagent (Mus musculus) | Nfkb2/p100 sgRNA KO2 | This paper | N/A | CTGTTCCACAATCACCAGAT |
| Recombinant DNA reagent (Mus musculus) | Map3k14/NIK sgRNA KO1 | This paper | N/A | TCAGAGCGCATTTTCATCGC |
| Recombinant DNA reagent (Mus musculus) | Map3k14/NIK sgRNA KO2 | This paper | N/A | GTCGAGGCAGTACCGGTCGC |
| Recombinant DNA reagent (Homo sapiens) | Traf3 sgRNA KO1 | This paper | N/A | AGATTCGCGACTACAAGCGG |
| Recombinant DNA reagent (Homo sapiens) | Traf3 sgRNA KO2 | This paper | N/A | CCTCACATGTTTGCTCTCGC |
| Recombinant DNA reagent (Homo sapiens) | DHB-mVenus | Spencer et al., 2013, PMID:24075009; Addgene | CAT: 136461, RRID:Addgene_136461 | |
| Recombinant DNA reagent (synthetic) | ES-FUCCI | Sladitschek and Neveu, 2015, PMID:25909630; Addgene | CAT: 62451, RRID:Addgene_62451 | |
| Recombinant DNA reagent (Mus musculus) | pWPI mScarlet NIK-ΔT3 | This paper | N/A | Mouse NIK with deleted TRAF3 binding motif (amino acids 78–84) cloned into pWPI-mScarlet vector |
| Antibody | Anti-NF2/MERLIN (rabbit monoclonal) | CST | CAT: 6995S, RRID:AB_10828709 | (1:750), WB |
| Antibody | Anti-TRAF3 (mouse monoclonal) | Santa Cruz | CAT: sc-6933, RRID:AB_628390 | (1:200), WB |
| Antibody | Anti-UBE2M (rabbit polyclonal) | Proteintech | CAT: 14520–1-AP | (1:500), WB |
| Antibody | Anti-NF-κB2 p100/p52 (rabbit polyclonal) | CST | CAT: 4882, RRID:AB_10695537 | (1:750), WB |
| Antibody | Anti-NIK (rabbit polyclonal) | CST | CAT: 4994S, RRID:AB_2297422 | (1:500), WB |
| Antibody | Anti-RELB (rabbit monoclonal) | Abcam | CAT: ab180127 | (1:1000), WB; (1:300), IF |
| Antibody | Anti-NF-kappaB Pathway Sampler Kit Antibody | CST | CAT: 9936, RRID:AB_561197 | (1:1000), WB |
| Antibody | Anti-CDKN1B/p27 (rabbit polyclonal) | BD Transduction Laboratories | CAT: 610241, RRID:AB_610241 | (1:1000), WB |
| Antibody | Anti-p21 (mouse monoclonal) | Invitrogen | CAT: ma5-14353, RRID:AB_10986834 | (1:1000), WB |
| Antibody | Anti-p19 (rabbit polyclonal) | Santa Cruz | CAT: sc-1063, RRID:AB_2078865 | (1:200), WB |
| Antibody | Anti-p18 (rabbit polyclonal) | Santa Cruz | CAT: sc-1064, RRID:AB_2078729 | (1:200), WB |
| Antibody | Anti-GAPDH (rabbit monoclonal) | CST | CAT: 2118S, RRID:AB_561053 | (1:2000), WB |
| Antibody | Anti-α-tubulin (mouse monoclonal) | Sigma-Aldrich | CAT: T-9026, RRID:AB_477593 | (1:4000), WB |
| Antibody | Anti-actin (mouse monoclonal) | Sigma-Aldrich | CAT: A4700, RRID:AB_476730 | (1:4000), WB |
| Antibody | Anti-BrdU antibodies (rat monoclonal) | Abcam | CAT: ab6326, RRID:AB_305426 | (1:800), IF, Flow |
| Antibody | Anti-YAP (rabbit polyclonal) | Novus Biologicals | CAT: NB110-58358, RRID:AB_922796 | (1:200), IF |
| Antibody | Anti-phospho-RB Ser807/811 (rabbit monoclonal) | CST | CAT: 8516S, RRID:AB_11178658 | (1:1000), IF |
| Antibody | Anti-CYCLIN D1 (mouse monoclonal) | Invitrogen | CAT: MA5-11387, RRID:AB_10987096 | (1:50), IF |
| Antibody | Anti-KI-67 (rabbit polyclonal) | Invitrogen | CAT: 18-0191Z, RRID:AB_86661 | 1:70, IF |
| Antibody | Anti-Phospho-HISTONE H3 (Ser10) (mouse monoclonal) | CST | CAT: 9706S, RRID:AB_331748 | 1:300, IF |
| Antibody | Anti-E-CADHERIN (rat monoclonal) | Thermo Fisher Scientific | CAT: 14-3249-80, RRID:AB_1210459 | 1:500, IF |
| Others | Hoechst 33342 Fluorescent dye | Life Technologies | CAT: 62249 | 1:1000, IF |
| Others | DAPI Fluorescent dye | Sigma-Aldrich | CAT: 422801 | 1:500, IF |
| Others | DRAQ5 Fluorescent dye | CST | CAT: 4084 | 1:1000, IF |
| Cell line (Mus musculus) | EpH4 | Dr. Jurgen Knoblich, Institute of Molecular Biotechnology, Vienna, Austria | Identity verified by RNAseq, DNA sequencing and immunofluorescent staining for epithelial markers | |
| Cell line (Homo sapiens) | HEK293T | ATCC | RRID:CVCL_0063 | |
| Cell line (Homo sapiens) | MCF10a | ATCC | RRID:CVCL_0598 | |
| Cell line (Mus musculus) | NIH 3T3 | ATCC | RRID:CVCL_0594 | |
| Strain (Mus musculus) | C3H/HeNCrl | Charles River Laboratories | CAT: CRL:025, RRID:IMSR_CRL:025 | |
| Chemical compound, drug | BV6 | ApexBio | CAT: B4653 | |
| Software, algorithm | GraphPad Prism | GraphPad | RRID:SCR_002798 | |
| Software, algorithm | CLC Genomics Workbench | QIAGEN | RRID:SCR_011853 | |
| Software, algorithm | Ingenuity Pathway Analysis (Qiagen) | QIAGEN | SCR_008653 | |
| Software, algorithm | Fiji is just ImageJ | N/A | RRID:SCR_002285 | |
| Software, algorithm | MAGeCK software | Li et al., 2014, PMID:25476604 | N/A |