Figure 5. Developmental expression of ZDHHC14 mirrors that of PSD93 and Kv1-type potassium ion channels in cultured hippocampal neurons.

(A) Hippocampal neurons were harvested at the indicated days in vitro (DIV) and lysates were western blotted with the indicated antibodies. (B) Quantified levels of the indicated proteins from A, relative to Histone H3 and normalized to DIV16 condition, plotted as a function of DIV (N = 3–4). Uncropped western blot images are in Figure 5—figure supplement 2.

Figure 5—figure supplement 1. Kv1 channel palmitoylated cysteine residues and surrounding regions are conserved.

(A) Schematic of a generic Kv1 channel subunit with transmembrane domains (purple) and palmitate attached to the palmitoyl-cysteine between transmembrane domains two and three. Residue numbers are indicated for the regions surrounding the palmitoyl-cysteine from rat Kv1.1 (top [Gubitosi-Klug et al., 2005], NP_775118.1). Corresponding regions of rat Kv1.2 and Kv1.4 are shown below (NP_037102.1 and NP_037103.1, respectively). (B) Residue numbers, indicated for the regions surrounding the palmitoyl-cysteine and the 10 C-terminal amino acids from Kv1.1 orthologs, including human Kv1.1 (H. sapiens; NP_000208.2), mouse Kv1.1 (M. musculus; NP_034725.3), rat Kv1.1 (R. norvegicus; NP_775118.1), western clawed frog Kv1.1 (X. tropicalis; XP_004912858.1), Zebrafish Kv1.1 (D. rerio; XP_005163101.1), fruit fly Shaker (Sh; D. melanogaster; NP_523393.3) and roundworm SHK-1 (C. elegans; NP_871935.1). In A and B conserved residues are highlighted in dark gray, functionally conserved residues are in light gray, transmembrane domain residues are outlined in dashed orange lines, and the palmitoyl-cysteine is red. In B PDZ-ligand consensus amino acids are in yellow.

Figure 5—figure supplement 2. Uncropped western blot images for Figure 5.

Bold titles indicate the figure that the uncropped images correspond to, boxes indicate cropped regions, and dashed lines and scissors indicate where membranes were cut prior to immunoblotting.