Skip to main content
. 2020 Nov 13;9:e56058. doi: 10.7554/eLife.56058

Figure 6. ZDHHC14 palmitoylates Kv1-type potassium channels in hippocampal neurons.

(A) Hippocampal neurons were transduced with the indicated lentiviruses on DIV9 and harvested on DIV16 to assess Kv1 channel palmitoylation by ABE. Palmitoyl-proteins (isolated by ABE; left panels) and total protein levels (in parent lysates; right panels) were assessed by western blotting with the indicated antibodies. (B) Quantified palmitoyl:total levels of Kv1.1, Kv1.2, and Kv1.4 from A, normalized to the control virus condition (Kv1.1: unpaired Student’s t-test ***p=0.0003, N = 10, 95% CI [0.18, 0.52]; Kv1.2: unpaired Student’s t-test *p=0.024, N = 10, 95% CI [0.047, 0.58]; Kv1.4: Mann Whitney test ****p<0.0001, N = 11, U = 0.0). (C) Quantified total levels of Kv1.1 (left), Kv1.2 (middle), and Kv1.4 (right) from A, normalized to the control virus condition (Kv1.1: unpaired Student’s t-test *p=0.019, N = 10, 95% CI [0.044, 0.43]; Kv1.2: unpaired Student’s t-test **p=0.0058, N = 9, 95% CI [0.087, 0.43]; Kv1.4: unpaired Student’s t-test ****p<0.0001, N = 11, 95% CI [0.25, 0.49]). Uncropped western blot images are in Figure 6—figure supplement 1.

Figure 6—source data 1. Source data for Figure 6B,C.

Figure 6.

Figure 6—figure supplement 1. Uncropped western blot images for Figure 6.

Figure 6—figure supplement 1.

Bold titles indicate the figure that the uncropped images correspond to, boxes indicate cropped regions, and dashed lines and scissors indicate where membranes were cut prior to immunoblotting.