Skip to main content
. 2020 Nov 3;130(12):6443–6456. doi: 10.1172/JCI126584

Figure 10. TNF-α induces downregulation of C-KIT and inhibits the pacemaker currents of isolated ICCs.

Figure 10

(A) C-KIT and CD34 immunofluorescence double staining was used to identify the ICC phenotype with or without TNF-α treatment. Green, CD34; red, C-KIT; blue, DAPI. Scale bars: 100 μm. (B) Pacemaker currents of ICCs were recorded using the whole-cell mode of the patch clamp technique. (C) Amplitudes of pacemaker currents with or without TNF-α treatment. Data shown represent results from 3 independent experiments. Student’s t test: ***P < 0.001.