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. 2020 Nov 3;130(12):6443–6456. doi: 10.1172/JCI126584

Figure 11. The TNF-α/NF-κB/miR-221 signaling pathway is involved in C-KIT expression in isolated ICCs.

Figure 11

(A) TargetScan software was used to predict the possible miRNAs that could target the 3′-UTR of c-Kit mRNA in humans and mice. (B) Western blotting of isolated ICCs with or without TNF-α and PDTC treatment. (C) Semiquantitative analysis of protein expression levels with each protein normalized to β-actin. (D) MiR-221 expression levels with or without TNF-α and PDTC treatment. (E and F) Western blot analysis of C-KIT and CD34 in isolated ICCs with or without TNF-α and miR-221 inhibitor treatment. Semiquantitative analysis of protein expression levels with each protein normalized to β-actin. Data shown represent results from 3 independent experiments. (G) The signaling pathway involving TNF-α, miR-221, and C-KIT in ICCs. One-way ANOVA: *P < 0.05; ***P < 0.001.