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. 2020 Nov 9;130(12):6677–6687. doi: 10.1172/JCI140189

Figure 2. Stress erythropoiesis cooperates with BCL11A enhancer editing to amplify γ-globin induction.

Figure 2

(A) Editing frequencies in granulocytes of ZM26 transplanted with BCL11A-edited CD34+ HPSCs. Slopes were calculated separately for the first 13 weeks of transplantation (early progenitor phase) and later time points (HSC phase) as indicated by the dashed line. (B) Distribution of monoallelic and biallelic edited colonies collected from methylcellulose plates for bone marrow mononuclear cells of ZM26 at 38 weeks after transplantation. (C) γ-globin protein expression in ZM26. The gray rectangle represents the phlebotomy course. (D) Hematocrit (HCT), (E) reticulocyte, and (F) γ-globin protein percentages in phlebotomized nontransplanted (ZL12 and ZM19), lentivirus-transduced (GFP vector–transduced) CD34+ HSPC-transplanted (RQ7278 and RQ7674), and BCL11A enhancer–edited animals (ZM26 and ZL25).