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. 2020 Nov 16;130(12):6639–6655. doi: 10.1172/JCI140046

Figure 6. β Cell membrane potential and insulin secretion from chronic high glucose–treated human islets.

Figure 6

(A) Representative traces of electrophysiological recordings of primary human β cells. Recordings were made in the presence of 1 mM and 10 mM glucose, following chronic (3 days) culture in normal glucose (5.5 mM), high glucose (16.7 mM), or high glucose (16.7 mM) plus YM-254890 (50 nM). (B) Membrane potential of primary human β cells. Recordings were made in the β cells under the same condition as described in A (n = 3–10 for each condition). (C) Insulin secretion from nondiabetic human islets. Insulin secretion in response to glucose following 3-day culture in 5.5 or 16.7 mM glucose is shown. Insulin secretion was normalized by the value at 2.8 mM glucose (=1) in the islets cultured in 5.5 mM glucose for 3 days (n = 13–14 for each condition). Pooled data from 4 independent experiments from 4 donors are shown. Statistical analyses were performed by 2-way ANOVA followed by Tukey’s post hoc test. Data are represented as mean ± SEM. *P < 0.05; **P < 0.01.