Figure 1.

ZO-1 expression is downregulated and ZONAB expression is upregulated in some bladder cancer cell lines. The expression of claudin-4 (a), ZO-1 (b), and ZONAB (c) protein in noncancer cell line (sv-huc-1) and bladder cancer cell lines (5637, UM-UC-3, and T24) was detected by Western blot. The relative grey value adjusted to the internal control β-actin and then standardized to the value of sv-huc-1 is shown by box and whisker plots. The whiskers represent the minimal or the maximal gray value, and the boxes span the interquartile range of measurements for cell lines with the mean value of 10 replicates (n = 10). ∗P < 0.05, one-way ANOVA, post hoc Tukey's test.