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. 2020 Nov 16;2020:6341256. doi: 10.1155/2020/6341256

Figure 2.

Figure 2

The expression of ZO-1 mRNA is downregulated and ZONAB mRNA is upregulated in bladder cancer (BC) cell lines and BC tissues. (a) The expression of claudin-4, ZO-1, and ZONAB mRNA in noncancer cell line (sv-huc-1) and bladder cancer cell lines (5637, UM-UC-3, and T24) was detected by real-time PCR. Relative expression adjusted to the reference gene GAPDH and then standardized to sv-huc-1 is shown by box and whisker plots. The whiskers represent the minimal or the maximal value and the boxes spans the interquartile range of measurements for cell lines with the mean value of 10 replicates (n = 10). ∗P < 0.05, one-way ANOVA, post hoc Tukey's test. (b) The expression of claudin-4, ZO-1, and ZONAB mRNA in BC tissue and adjacent nontumor tissues (ANTT) was detected by real-time PCR. Relative expression adjusted to the reference gene GAPDH and then standardized to the value of ANTT is shown by box and whisker plots. The whiskers represent the minimal or the maximal value, and the boxes span the interquartile range of measurements for 53 patients with the mean value of 3 replicates (n = 53).∗P < 0.05, Student's t-test.