Figure 1.

Identification of bone marrow mesenchymal stem cells. (a) BM-MSC express specific surface markers. After being cultured on the 5th generation, BM-MSC from healthy volunteers were stained with FITC-conjugated anti-CD105, CD44, and CD45 and PE-conjugated CD90, CD73, CD29, and CD34 monoclonal antibodies and appropriate controls. (b) The osteogenic and adipogenic differentiation of BM-MSC. The BM-MSC of the same generation were cultured with normal complete medium (adding b-FGF to help keep multipotent), osteogenic medium, or adipogenic medium. MSC differentiated into osteocytes characterized by Alizarin red staining (middle, 40x) and adipocytes characterized by lipid vacuoles (right, 100x).