Figure 3.

RIPK3-induced necroptosis is mediated by SIRT3 and SIRT6. (a) LNCaP cells stabilized expressed shSIRT3 and shSIRT6 with recombinant GFP and were treated with TNF (20 ng/ml). Cell death was tracked by staining with cytotoxic red and monitored by Incucyte. (b) Cell death curve of LNCaP, PC3, and DU145 cells after induction of control, SIRT1, SIRT3, or SIRT6 shRNA expression with doxycycline. (c) Western blot analysis of p-RIPK3 and p-MLKL protein levels in LNCaP and PC3 cells treated with 20 ng/ml TNF. (d) Cell death curve of LNCaP cells treated by RIPK3 inhibitor GSK′872 after induction of control, SIRT3, or SIRT6 shRNA expression with doxycycline.